Abstract

Background: The condition of hyperlipidemia can lead to the occurrence of oxidative stress which is characterized by the increasing number of Reactive Oxigen Species (ROS). ROS will activate the Nuclear Factor-Kappa B (NF-κB) signaling pathway. Oxidative stress and inflammatory conditions can be inhibited by natural ingredients that contain antioxidants and antiinflammatory. Antioxidant of caffeine can reduce and protect cells from free radicals such as ROS, then the progression of atherosclerosis can be inhibited. Objective: To determine the effect of Robusta coffee (Coffeacanephora) on NF-κB expression in male wistar coronary arteries induced by high-fat diet. Methods: Fifteen male wistar rats were divided into three groups: control group, hyperlipidemia group, and coffee treatment group. High fat diet for the hyperlipidemia group is made by mixing yolks of duck and pork oil. Infraorbital blood was taken for examination of LDL levels and rats were sacrificed at the 29th day, heart organs then carried out for tissue processing histologically. Observation and measurement of NF-κB expression performed by immunohistochemically. Results: The results showed that the coffee treatment group expressed NF-κB in coronary artery endothelial cells was less than the hyperlipidemia group (p